prikaz prve stranice dokumenta Analiza sadržaja DNA u celomocitima trpa Holothuria tubulosa protočnom citometrijom
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undergraduate thesis
Analiza sadržaja DNA u celomocitima trpa Holothuria tubulosa protočnom citometrijom
2015. urn:nbn:hr:137:203827
Jeremić, Vanja
University of Pula
Faculty of Natural Sciences

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Jeremić, V. (2015). Analiza sadržaja DNA u celomocitima trpa Holothuria tubulosa protočnom citometrijom (Undergraduate thesis). Pula: University of Pula. Retrieved from https://urn.nsk.hr/urn:nbn:hr:137:203827

Jeremić, Vanja. "Analiza sadržaja DNA u celomocitima trpa Holothuria tubulosa protočnom citometrijom." Undergraduate thesis, University of Pula, 2015. https://urn.nsk.hr/urn:nbn:hr:137:203827

Jeremić, Vanja. "Analiza sadržaja DNA u celomocitima trpa Holothuria tubulosa protočnom citometrijom." Undergraduate thesis, University of Pula, 2015. https://urn.nsk.hr/urn:nbn:hr:137:203827

Jeremić, V. (2015). 'Analiza sadržaja DNA u celomocitima trpa Holothuria tubulosa protočnom citometrijom', Undergraduate thesis, University of Pula, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:137:203827

Jeremić V. Analiza sadržaja DNA u celomocitima trpa Holothuria tubulosa protočnom citometrijom [Undergraduate thesis]. Pula: University of Pula; 2015 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:137:203827

V. Jeremić, "Analiza sadržaja DNA u celomocitima trpa Holothuria tubulosa protočnom citometrijom", Undergraduate thesis, University of Pula, Pula, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:137:203827

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Metadata
TitleAnaliza sadržaja DNA u celomocitima trpa Holothuria tubulosa protočnom citometrijom
Title (english)Flow cytometric analysis of DNA content in Holothuria tubulosa coelomocytes
AuthorVanja Jeremić
MentorMaja Fafanđel (mentor)
Committee memberRenato Batel (predsjednik povjerenstva)
Committee memberMaja Fafanđel (član povjerenstva)
Committee memberMirta Smodlaka Tanković (član povjerenstva)
Committee memberNastjenka Stupić (član povjerenstva)
GranterUniversity of Pula
(Faculty of Natural Sciences)
Pula
Defense date and country2015-07-22, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Interdisciplinary Natural Sciences
Marine Science
Abstract
U ovom radu su istraživani celomociti trpa Holothuria tubulosa protočnom citometrijom. Analizom sadržaja DNA ustanovljeno je da se celomociti trpa dijele u celomskoj šupljini. Normalan stanični ciklus je određen u jedinkama s referentne postaje. U pojedinim jedinkama s područja izloženih antropogenom utjecaju utvrđene su promjene staničnog ciklusa i to: zastoj G1 faze ("G1-arrest"), odgoda G2 faze ("G2-delay") i apoptoza. Analiza utvrđenih učinaka je pokazala da rovinjska luka nije područje pod utjecajem zagađivala dok je požar na brodu UND Adriyatik imao utjecaja na faunu okolnog podmorja. Rezultati ovog istraživanja potvrđuju da se H. tubulosa može koristiti kao bioindikatorski organizam u procjeni onečišćenja mora, odnosno da je analiza celomocita protočnom citometrijom dobar pokazatelj stanja u morskom ekosustavu.
Abstract (english)
In this study, the coelomocytes of Holothuria tubulosa were analized by flow cytometry. The DNA content analyses identified coelomocytes proliferation in the coelom. The pattern of a normal cell cycle has been determined in individuals from the reference site. In some individuals from sites under anthropogenic influence cell cycle alteration were detected: "G1-arrest", "G2-delay" and apoptosis. Analysis of the extent of the effects revealed that the port of Rovinj is an area with no impact while the fire damage on the UND Adriyatik ship caused a negative consequences to the surrounding fauna. The results of this study suggest that Holothuria tubulosa could be a useful bioindicator for pollution monitoring, while flow cytometric analyses of Holothuria coelomocytes could provide information regarding the marine ecosystem status.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:137:203827
Study programmeTitle: University Undergraduate Study Programme - Marine Sciences
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) znanosti o moru (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) znanosti o moru)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2017-03-20 11:56:39