Regional policy actors present key players in the implementation of the regional policy measures and realization of the defined policy goals. Lately, their importance and the need for coordinated cooperation at different levels of government in the Republic of Croatia have become more highlighted due to the fact that regions have higher opportunities, especially after the EU accession. The aim of this final thesis is to present the role of chosen regional policy actors in Croatia. After defining the main characteristics of regional policy, regional policy actors are described, such as the Ministry of Regional Development and EU Funds, households, entrepreneurs, development agencies and local action groups. More detailed analysis of the activities and performances of regional policy actors in Istria County has confirmed their important role in fostering regional development. There are also determined the need and benefits of the implementation of the partnership and cooperation as the main principles within the regional policy, regarding that joint actions of public, private sector and civil society partners, at national, regional and local level can lead to multilevel positive effect in managing development and to create specific, recognizable regional economies.