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undergraduate thesis
Utjecaj benzo(a) pirena na integritet DNA-a škrgama dagnje M. galloprovincialis
2020. urn:nbn:hr:137:142922
Anić, Dorotea
University of Pula
Faculty of Natural Sciences

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Anić, D. (2020). Utjecaj benzo(a) pirena na integritet DNA-a škrgama dagnje M. galloprovincialis (Undergraduate thesis). Pula: University of Pula. Retrieved from https://urn.nsk.hr/urn:nbn:hr:137:142922

Anić, Dorotea. "Utjecaj benzo(a) pirena na integritet DNA-a škrgama dagnje M. galloprovincialis." Undergraduate thesis, University of Pula, 2020. https://urn.nsk.hr/urn:nbn:hr:137:142922

Anić, Dorotea. "Utjecaj benzo(a) pirena na integritet DNA-a škrgama dagnje M. galloprovincialis." Undergraduate thesis, University of Pula, 2020. https://urn.nsk.hr/urn:nbn:hr:137:142922

Anić, D. (2020). 'Utjecaj benzo(a) pirena na integritet DNA-a škrgama dagnje M. galloprovincialis', Undergraduate thesis, University of Pula, accessed 11 November 2024, https://urn.nsk.hr/urn:nbn:hr:137:142922

Anić D. Utjecaj benzo(a) pirena na integritet DNA-a škrgama dagnje M. galloprovincialis [Undergraduate thesis]. Pula: University of Pula; 2020 [cited 2024 November 11] Available at: https://urn.nsk.hr/urn:nbn:hr:137:142922

D. Anić, "Utjecaj benzo(a) pirena na integritet DNA-a škrgama dagnje M. galloprovincialis", Undergraduate thesis, University of Pula, Pula, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:137:142922

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Metadata
TitleUtjecaj benzo(a) pirena na integritet DNA-a škrgama dagnje M. galloprovincialis
Title (english)Effect of benzo(a) pyrene on DNA integrity in gills of mussel M. galloprovincialis
AuthorDorotea Anić
MentorMaja Fafanđel (mentor)
MentorInes Kovačić (sumentor)
Committee memberEmina Pustijanac (predsjednik povjerenstva)
Committee memberMaja Fafanđel (član povjerenstva)
Committee memberNevenka Bihari (član povjerenstva)
Committee memberInes Kovačić (član povjerenstva)
GranterUniversity of Pula
(Faculty of Natural Sciences)
Pula
Defense date and country2020-09-17, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Interdisciplinary Natural Sciences
Marine Science
Abstract
U ovom radu istražen je učinak benzo(a)pirena (B(a)P) na integritet DNA u škrgama dagnje Mytilus galloprovincialis pomoću Fast mikrometode. Ta metoda detektira smanjenje integriteta DNA uslijed oštećenja pomoću fluorokromatskog bojila PicoGreen koji ima sposobnost vezanja za dvostruku DNA u alkalnim uvjetima. Jedinke dagnje su izlagane različitim koncentracijama B(a)P-a u vodi nakon čega je utvrđen dozni odgovor ukazujući na genotoksični učinak B(a)P-a u škrgama dagnje. Koncentracije B(a)P-a u vodi veće od 0.1 μg/L rezultiraju statistički značajnim smanjenjem integriteta DNA u škrgama.
Abstract (english)
In this work, the effect of benzo (a) pyrene (B (a) P) on DNA integrity in the gills of the mussel Mytilus galloprovincialis was investigated using the Fast micromethod. This method detects a decrease in DNA integrity due to damage using the fluorochromatic dye PicoGreen which has the ability to bind to double DNA under alkaline conditions. Mussel specimens were exposed to different concentrations of B (a) P in seawater after which a dose response was determined indicating the genotoxic effect of B (a) P in mussel gills. Concentrations of B (a) P in water greater than 0.1 μg / L result in a statistically significant reduction in DNA integrity in gills.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:137:142922
Study programmeTitle: University Undergraduate Study Programme - Marine Sciences
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) znanosti o moru (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) znanosti o moru)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-09-28 09:13:56