Two-part narrow-interval singing of Istria and the Croatian Littoral presents a phenomenon of intangible cultural heritage of exceptional importance. As a complex folklore style, from 2009. it has been on the UNESCO's representative list of intangible cultural heritage of humanity. It is most intensely practised in Istria, the Croatian Littoral and the island Krk. Two-part narrow-interval singing contains several genres and styles of performance: kanat, tarankanje, bugarenje and discant. Specific instruments are sopele (roženice or sopile), mih, pive, šurle and dvojnice among aerophones, and cindra as a chordophone. Apart from folklore ensembles, Istrian two-part singing is performed in various circumstances, through informal gatherings, funerals, weddings, celebrations, in ethno music and everyday life. In a tradition intertwined with the continuity of change, it is evident that musicians resort to the use of artifical materials to make musical instruments and mouthpieces. There's also a significant number of virtuosos who use two-part singing outside the standardized rules and apply their own personal experiences to popularize it. The livelines of Istrian two-part singing is shown in this paper through the example of musicians and singers from a younger generation, prominent virtuosos, the work of folklore ensembles and through atypical expressions that confirm it as a living and authentic experience of the present imbued with the past.