This thesis deals with the socio-cultural influents of the touroperators on the tourist destination and the process of the change of unsustainable tourism to sustainable and responsible. Namely, the package holidays revolutionized tourist traveling what made the touroperators synonym for the mass tourism. In the initial stages of the development of modern tourism that synonym has a positive connotation, but later started to receive a negative one. That is primarily due to the bad impact on the environmental and socio-cultural conditions of the tourist especially destinations in developing countries. In these destinations gripped hegemony with tourism enclaves which provoked a number of negative effects such as demonstration effect, acculturation, stereotypes, the commodification of culture, antagonism and others. However, such an unsustainable development of tourism touroperators then differentiation of product and market segmentation replaced by tourism of special interests based on sustainable development that harmonize tourism with economic, ecological and socio-cultural sphere of destinations. Therefore, the quantity is replaced by the quality. But there are always deviations from the concept of sustainable development because the concept describes ideal image. Ideal image in practice is not always feasible, but still serves as an orientation and the goal should be to get as close as possible to the ideal situation. The paper presents the examples of the positive and negative touroperator's impact on the socio-cultural enviroment of the destinations. The results of the analysis shows that the touroperators today are significantly improving the socio-cultural conditions in the local community with the eco-tourism and creative-cultural tourism offer and at the same time allowing tourists new activities and experience.