The importance of non-profit organizations nowadays is greatly demonstrated by the fact that they are seen as bearers of the civil society. One of the current issues is the non-profit accounting and non-profit accounting information system. In the years 2014 and 2015 new laws and regulations have been adopted which are directed toward nonprofit organizations. Adopted legislation refers to the multiple aspects of non-profit organizations. The limit for determining the double-entry bookkeeping obligor was raised to 230,000.00 kuna, non-profit organization shall use double-entry bookkeeping three years since its establishment, while the number of ledgers in a single-entry bookkeeping increased. The law introduces the obligatory submission of financial statements to all non-profit organizations, but adjusted to the amount of income and assets. Publicly disclosed financial statements are available in the Register of non-profit which contributes to the principle of transparency. A non-profit organization which uses double-entry bookkeeping is obliged to carry out a self-assessment of effective and efficient functioning of financial management and control. Non-profit organizations which use double-entry bookkeeping must by the end of the year draw up a work program and financial plan for the next period. In this thesis the author will try to answer the question how and to what extent does the new legislation affect the accounting information system of non-profit organizations, both in theory and in practical examples. This paper presents the novelties that should contribute to establishing a system of sound financial management and control, both in accounting and in programming, preparing financial plans and their realization and reporting. Also, there are certain solutions offered which are directed towards the development of quantitative indicators for non-profit organizations, as well as suggestions for improvement of accounting software for non-profit sector. Through the case study, the humanitarian organization Red Cross Society of Pula was analyzed, to see how legislative changes affect the accounting information system in practice. Under the influence of globalization, non-profit organizations are transformed from organizations that operate strictly localy, toward the ones with the regional, even global reach. With this in mind, as a follow-up question in this paper the topic of the role and usage of the English language in the accounting information system of non-profit organizations is discussed.