This work explores the multifaceted role of port cities in the realm of culture, with a particular focus on the European Capital of Culture (ECoC) project. The study aims to understand how port cities navigate cultural, economic, and social challenges through the lens of urban regeneration and ECoC initiatives. Utilizing a multi-disciplinary approach, the research delves into historical contexts, various models of urban regeneration, and the specific challenges and opportunities that port cities face. The methodology involves a comparative analysis of multiple port cities that hosted ECoC events, as well as case studies and interviews with key stakeholders. This comprehensive approach allows for a nuanced understanding of how the ECoC project impacts host cities in various dimensions, including economic revitalization, social inclusion, and environmental sustainability. Key findings suggest that the ECoC project offers a unique platform for port cities to engage in urban regeneration, fostering both cultural enrichment and socio-economic development. However, the success of these initiatives is contingent upon adaptive governance and community involvement. The study concludes that port cities can leverage ECoC projects as a catalyst for holistic urban regeneration, transforming themselves into vibrant, sustainable, and inclusive communities. Nonetheless, this transformation requires a nuanced understanding of local challenges and the active participation of a diverse set of stakeholders. By providing a comprehensive analysis of port cities in the context of cultural projects and urban regeneration, this thesis contributes to the growing body of literature on the subject and offers actionable insights for policymakers, urban planners, and community leaders.