Adolescence is a developmental stage during which individuals begin to strengthen a sense of identity, characterized by autonomous functioning that will lead to independent living while maintaining secure relationships with family, peers and the wider community, and sexual maturation. The aim is to examine and compare knowledge, attitudes and habits about adolescent sexuality, and to determine whether there are differences depending on whether the students are from medical school or high school. The analysis was conducted with an anonymous questionnaire of 27 general questions, as well as questions of habits, behavior, knowledge and attitudes related to sexuality. The research included students of the Secondary Medical School and Gymnasium "Matija Mesić" in Slavonski Brod aged 17 to 20 years. 100 students participated; 72 girls and 28 boys. The obtained data show that 59% of the respondents had sexual intercourse. Only 17% of respondents showed a high level of knowledge related to sexuality and prevention of risky behaviors. The majority of respondents (65%) believe that free contraceptives would reduce the number of unwanted pregnancies and sexually transmitted diseases, while almost all (91%) believe that health education is needed in schools. and the most common reason for having sex for the first time is love. Almost all respondents use contraception. Respondents' knowledge of sexuality, especially high school students, is at an unsatisfactory level. Respondents have much clearer views against abortion than male respondents. Most respondents want health education in schools.