Mussel Mytilus galloprovincialis is one of the best biological indicators for biomonitoring of marine ecosystems. It is used to assess pollution in the coastal area due to its sessile nature and filter feeding and the accumulation of chemical contaminants from seawater. Continuous anthropogenic pressure along the shores of all, including the Adriatic Sea, results in the constant introduction of a wide range of different chemicals into the marine environment. These chemicals affect the health of marine ecosystems. By direct discharge, through precipitation or soil leaching, most wastes of anthropogenic origin most often end up in inland waters and the sea, which thus become the final recipients of most pollutants and are thus particularly vulnerable. Chemicals accumulate in organisms and have the potential to induce changes at the level of molecules, cells and tissues, providing various warning signals of events that could occur at the level of populations, communities and ecosystems. anthropogenic influence that affects the change in the physiological state of mussels caused by stress, it is necessary to analyze: fitness index, hepatosomatic index and air survival. Mussels of the species Mytilus galloprovincialis were sampled from 6 locations to determine which locations are most affected by anthropogenic pressures and to describe and determine whether there is an interdependence of selected parameters and whether these parameters can be used to differentiate anthropogenic pressures. It was established that the fitness index, hepatosomatic index and air survival maintain anthropogenic pressures at certain locations, and the highest anthropogenic pressure was observed at the locations of the Port of Pula and the Port of Rijeka. Since no interdependence of the selected parameters was observed and due to the adaptation of mussels to specific stress conditions, it can be concluded that the selected parameters cannot be used to differentiate the specific anthropogenic pressure.