Tourism and eno-gastronomy are two very closely related concepts whose product and service offer is part of the culture of the locality that visitors meet and get to know during their arrival and stay in the tourist destination during the consumption of food and drink, by participating in various activities and thematic manifestations and events. The rural area represents the basic prerequisite for the development of wine tourism with a high level of tourist potential as a source of entrepreneurial opportunities and ideas for the initiation of entrepreneurial ventures that, through their implementation, will influence the development of tourism and the economy in the tourist destination. The approach to the entrepreneurial initiative and the implementation of ventures in wine tourism should, in addition to the basis, which is primarily made up of the quality of the wine; include the authenticity of the area, event management and connect the tourist dimension connected with the provision of accommodation in order to create a unique ambiance in wine tourism. The development of entrepreneurship in wine tourism is important for the development of the image of the tourist destination, the entrepreneurial environment and climate, and for meeting the increasingly complex needs of modern tourist demand.