Due to increased scientific and general public interest of genotoxin effects in the environment, bioremediation of the toxicants and further investigation of a vast spectrum of bioactive compounds is needed. Bioremediation is a process in which metabolic potential of microorganisms, such as bacteria and fungi, is used to cleanse contaminated areas and materials. Metalloenzyme laccase is a frequent and widespread enzyme of the oxidoreductase group and his primary function is degradation of lignin in fungi. Laccase is not specific to a substrate nor is it stereospecific. Bioremediation effect of the lactase enzyme was tested by incubation of lactase with different dilutions of model compounds and determination of genotoxic activity by umu-test. Genotoxic potential, in other words, genotoxic activity, was determined by measuring β-galactosidase activity and calculating induction ratio (IR), while the bioremediation effect was observed through comparison of genotoxic activity of model compounds concentrations with and without laccase. Based on the results of diminished genotoxic activity of model compounds 4-NQO, 2-AA and the mixture of hydrocarbons present in the water soluble fraction of crude oils (WSF). From presented data it is possible to conclude that laccase concentration of 1 U/ml has a potentially bioremediative value and does not possess metabolic activity which could be used for metabolic activation of indirect genotoxins instead of the S9 liver fraction. This is a preliminary experiment and further examinations and optimization of conditions are needed regarding both the enzyme and determination of activity by umu-test.