Despite their small size, ponds are extremely important for biodiversity. They provide a refuge to endemic species of flora and fauna and are especially important for migratory birds. Medulin pond is located in the southwestern part of the municipality of Medulin on the Istrian peninsula. The pond is surrounded by a moderately urbanized settlement and is under a moderate anthropogenic influence. The aim of this research was to assess the ecological and sanitary conditions of the Medulin pond in order to preserve it and to make full use of its multiple purposes in forms of recreation. For research purposes, the geodetic measurement, analysis of hydrographic parameters and macrofauna of the pond were carried out. Samples were collected for the analysis of the microbial community, sanitary quality of water and sediment. The depth of the pond, which was unknown until now, was obtained by geodetic measurement. The pond ecosystem is characterized by high primary productivity and high oxygen saturation. Microbiological parameters measured in the surface water of the Medulin pond indicate an increased number of microorganisms, while the analysis of sanitary quality indicators displayed a moderate contamination, which qualifies the water as good or excellent. Analysis of the macrofauna revealed a high level of biodiversity, and in recent years a reintroduction of the dragonfly population has been observed, which indicates a high-quality and rich ecosystem. This research can serve as a basis for further research.