The County of Istria is one of the most prominent in Croatia in terms of the level of women's involvement in entrepreneurship in Croatia. This is supported by the fact that entrepreneurship in Istria is one of the most developed. In contrast, Međimurje County is characterized by a lower degree of women engaged in entrepreneurial activities, primarily due to limited supply of female labor in the labor market of Međimurje, but also weaker supply in the labor market. The aim of the final paper is to present the determinants and problems of women's entrepreneurship in theory and to present a comparative analysis of women's entrepreneurship in Istria and Međimurje counties. The purpose of this paper is to point out the importance of implementation and engaged women in entrepreneurship and to point out the development differences of women's entrepreneurship in these two counties. Removing barriers, such as discriminatory property and inheritance laws, discriminatory customary laws, poor access to formal financial institutions, and time constraints due to family and household responsibilities, could create greater opportunities for a sustainable women-run enterprise.